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Sulfonamides (SAs) ELISA Kit
Catalog No. LSY-10009
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Sulfonamides residue. The coupling antigens are pre-coated on the micro-well stripes. The Sulfonamides in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfonamides antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfonamides in the sample. This value is compared to the standard curve and the Sulfonamides concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.4 ppb
Incubator temperature: 25℃
Incubator time: 60min～20min
Detection limit
Tissue (high-detection-limit method)............................................... 0.4 ppb
Tissue (lower-detection-limit method)……………………………………...2 ppb
Honey………………………………….……………………………………0.4 ppb
Serum, urine................................................................................. 1.6 ppb
Milk................................................................................................. 8 ppb
Cross-reaction rate
Sulfadiazine (SD or SDZ)................................................................. 100%
Sulfamonomethoxine (SMM)........................................................... 97.6%
Sulfamethoxazole (SMZ).................................................................. 100%
Sulfathiazole (ST).......................................................................... 195.0%
Sulfamerazine (SM1)........................................................................... 92%
Phthalylsulfathiazole................................................................. 73% (PST)
Sulfamethyltiadiazolum..................................................................... 110%
Sulfapyridine..................................................................................... 48%
Sulfamethoxydiazine(SMD)............................................................. 26.4%
Sulfafurazole(SIZ).............................................................................. 95%
Sulfanethazine(SM2)………………………………………………………. 140%
Sulfadimethoxine(SDM)…………………………………………………… 110%
Sulfadoxine (SDM2)…………………………………………………………..80%
Sulfaquinoxaline(SQX)………………………………………………………160%
Sulfamethoxypyridazine (SMP)………………………………………………80%
Sulfachlorinepyridazine (SPDZ)……………………………………………...75%
Sulfabenzoyl (SML)…………………………………………………………..104%
Conclusion drawn from the cross-reaction rate: This kit can be used for the test of Sulfonamides, totally meet the national testing demand of Sulfonamides.
Recovery rate
Tissue, honey............................................................................. 85±25%
Urine, milk, serum........................................................................ 70±20%
3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 6× standard solution (1 mL each): 0 ppb, 0.4 ppb, 1.2 ppb, 3.6 ppb, 10.8 ppb and 32.4 ppb
3） Enzyme conjugate (7 mL)............................................................... red cap
4） Antibody working solution (10 mL)................................................ blue cap
5） Substrate A solution (7 mL)......................................................... white cap
6） Substrate B solution (7 mL)......................................................... black cap
7） Stop solution (7 mL).................................................................. yellow cap
8） 20× concentrated washing buffer (40 mL)..................................... white cap
9） 2× concentrated redissolving solution (50 mL)..................... transparent cap
