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Nitrofuran (AOZ) ELISA Test Kit

Nitrofuran (AOZ) ELISA Test Kit
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FOB Price: Negotiable 
Product Type: Biotech 
Port: Shenzhen 
Minimum Order Quantity:  
Supply Ability:
Place Of Origin: Shenzhen,China
Delivery Time: 3 days
Expiry Date: long-term effectiveness
Last Update: 2015-09-09
Views: 208
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Product Details
 

Nitrofuran (AOZ) ELISA Test Kit
Catalog No. LSY-10002
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained.
 
2. Technical specifications
Sensitivity:  0.02ppb
Incubation Temperature:  25
Incubation Time:  60min30min
Detection limit
Tissue, liver................................................................................ 0.04 ppb
Honey, milk, intestine................................................................... 0.04 ppb
Fish, shrimp (some interference).................................................... 0.1 ppb
Cross-reaction rate
AOZ................................................................................................ 100%
AMOZ............................................................................................ <0.1%
AHD.............................................................................................. <0.1%
SEM.............................................................................................. <0.1%
Recovery rate
Tissue, liver.................................................................................. 80±25%
Honey, milk, intestine.................................................................... 75±17%
 
3. Components
1)     Micro-well strips: 12 strips with 8 removable wells each
2)     6× standard solution (1 mL each): 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb and 1.62ppb
3)     Enzyme conjugate (7 mL).............................................................. red cap
4)     Antibody working solution (7 mL)................................................. blue cap
5)     Substrate A solution (7 mL)........................................................ white cap
6)     Substrate B solution (7 mL)........................................................ black cap
7)     Stop solution (7 mL)................................................................. yellow cap
8)     20× concentrated washing buffer (40 mL).................................... white cap
9)     2× concentrated redissolving solution (50 mL).................... transparent cap
10) 2-Nitrobenzaldehyde (C7H5NO3)(10 mL)........................................ white cap
 
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