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Zearalenone ELISA test kit

Zearalenone ELISA test kit
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FOB Price: 350.00 USD/kit  
Product Type: Biotechnology 
Port: Shenzhen 
Minimum Order Quantity: 1 kit 
Supply Ability: 1000 kit
Place Of Origin: China
Delivery Time: 3 days
Expiry Date: long-term effectiveness
Last Update: 2015-09-09
Views: 281
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Product Details
 

Zearalenone ELISA test kit

                                                                           Catalog No. LSY-10030

1. General

Zearalenone (ZEN), also called as F-2 toxin, the main component of 2,4 - dihydroxybenzoic acid ester compounds produced by Fusarium graminearum and yellow sickle monocytogenes. ZEN mainly contaminate corn, wheat, grain and other crops, which has a strong estrogen effect, and can cause estrogen pigs, rats, mice, poultry and other animals too much disease and severe symptoms of reproductive tract and infertility, also has immunotoxic, genotoxic etc., it has estrogen effect to human too.

Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Zearalenone detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Zearalenone ELISA kit to analyze Zearalenone residue of samples is fast and accurate.

This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.

2. Test principle

This test kit is based on the competitive enzyme immunoassay for the detection of Zearalenone in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Zearalenone in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Zearalenone antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Zearalenone in it. This value is compared to the standard curve and the Zearalenone concentration is subsequently obtained.

3. Application

This test kit can be used to detect Zearalenone in grain, beer and feed samples qualitatively and quantitatively.

4.  Technical specifications

Sensitivity:  0.2 ppb

Detection limit:

Grain ……………..…………………………………………..……..  2 ppb

Beer  ………………………………………………………..………..2 ppb

Feed …………………………………………………………..……….40 ppb

Cross-reaction rate:

Zearalenone........................................................................... …..100%

α-zearalanol.................................................................................. 75%

β-zeranol...................................................................................... 61%

α-Zearalenol ............................................................................. 103%

β-Zearalenol ............................................................................... 83%

Zearalanone ................................................................................ 75%

5.  Components

1)  ZEN Micro-well strips: 12 strips with 8 removable wells each

2)  ZEN 6 x standards ………………………………………2ml each

0 ppb (zero standard), 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb

3)  ZEN Enzyme labled conjugate...........................................7 ml

4)  Substrate................................................................ ..........12 ml

5)  Stop solution..................................................................... 10 ml

6)  Concentrated wash buffer (10).........................................50ml

7) Concentrated sample diluent (10).................................... 20ml 
 
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