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CommaX Nucleic Acid Purification Spin Columns (0-30 Ug, Capped Outer Tube)
NP30 NP30-A CommaPrep® Plasmid Miniprep Columns
The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 30 μg.
CommaPrep® plasmid miniprep columns are supplied with spin columns and collection tubes.The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 30 μg.
Extracted plasmid is ready for use in many downstream applications, including restriction digestion, ligation and transformation, PCR, sequencing and library construction.
Features
- Up to 30 μg high purity plasmid DNA
- High quality with reproducible yields
- Single protocol for high or low copy plasmids
- Suitable for various downstream applications
Typical results
pUC19 was extracted from 3 mL overnight E.coli (DH5a) using columns made by comapy A, company T and Biocomma, and the elution volume was 80 μL. Meanwhile, extracted plasmid pUC19 from 10 mL overnight E.coli (DH5a) using Biocomma HP50, and the elution volume was 100 μL. Then analyze by electrophoresis on a 1% agarose gel.
Lane 1: Column from company A
Lane 2: Column from company T
Lane 3: Biocomma NP30
Lane 4: Biocomma HP50
Figure 1: Plasmid DNA extracted using various columns
Experimental overview
Table 1: The yield of plasmid DNA extracted using various columns
| A260/280 | Concentration (ng/μL) | Yield (μg) | |
|---|---|---|---|
| Column from company A | 1.92 | 312 | 25 |
| 1.96 | 300 | 24 | |
| Column from company T | 1.86 | 350 | 28 |
| 1.82 | 325 | 26 | |
| Biocomma NP30 | 1.91 | 350 | 28 |
| 1.88 | 337 | 27 | |
| Biocomma HP50 | 2.01 | 800 | 80 |
| 2.03 | 850 | 85 |
Order information:
| Cat. # | Description | Volume | Yield | Qty. |
|---|---|---|---|---|
| NP30 | CommaPrep® Plasmid Miniprep Columns,Capless spin columns, Blue fixing rings | 2 mL，800 μL | ~30 μg | 500/Box |
| NP30-A | CommaPrep® Plasmid Miniprep Columns,Capped spin columns, Blue fixing rings | 2 mL，800 μL | ~30 μg | 500/Box |
| NP30-AS | CommaPrep® Plasmid Miniprep Columns,Capped spin columns, White fixing rings | 2 mL，750 μL | ~30 μg | 500/Box |
| MNP001-1 | CommaXP® Plasmid Miniprep Kit | 50 Preps | ||
| MNP016-1 | CommaXP® Rapid Plasmid Miniprep Kit | 50 Preps | ||
Note: It is recommended that the elution volume should be 60-100 μL by using series of NP30.
