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CommaX Nucleic Acid Purification Spin Columns (0-100 Ug)
NP100 CommaPrep® Plasmid Midiprep Columns
The columns are based on silica membrane technology and provide fast and reliable extraction of high quality plasmid with a binding capacity of 100 μg (NP100) or 200 μg (HP200).
CommaPrep ® plasmid midiprep columns are supplied with spin columns andcollection tubes.The columns are based on silica membrane technology andprovide fast and reliable extraction of high quality plasmid with a bindingcapacity of 100 μg (NP100) or 200 μg (HP200).
Extracted plasmid is ready for use in many downstream applications, includingrestriction digestion, ligation and transformation, PCR, sequencing and libraryconstruction.
Features
- Up to 100-200 μg high purity plasmid DNA
- Simple procedures and easy to operate
- Single protocol for high or low copy plasmids
- Suitable for various downstream applications
Typical results
Use NP100 to extract pET32a expression plasmid,and verify with double enzyme digestion.
Lane 1：Plasmid pET32a
Lanes 2-3：Plasmid by restriction digestion
Figure 1: Plasmid DNA analysed by restriction digestion
Experimental overview
Order information:
| Cat. # | Description | Volume | Yield | Qty. |
|---|---|---|---|---|
| NP100 | CommaPrep ® Plasmid Midiprep Columns | 15 mL，4 mL | ~100 μg | 50/Box |
| HP200 | CommaPrep ® High-yield Plasmid Midiprep Columns | 15 mL，4 mL | ~200 μg | 50/Box |
| MNP001-3 | CommaXP ® Plasmid Midiprep Kits | 10 Preps | ||
Note: It is recommended that the bacteria culture should be 30-50 mL and the elution volumeshould be 400-800 μL by using CommaPrep ® plasmid midiprep columns.
